Determination of IgG in Serum and Egg Yolk of Goose

Zhiyuan Ai

Abstract


Immunoglobulin G (IgG) refers to animal protein with antibody activity. In poultry, the mother can pass their own immunoglobulin to the off spring through the egg, and play a protective role for the off spring [20]. In this study, 102 samples of Jilin white goose were tested by ELISA. The IgG content of IgG and goose embryo in egg yolk during the development of goose embryo was tested. The results showed that the IgG content in goose serum and egg yolk was 14.707μg / mL and 3967.62μg / mL at 14.5 days, and 1495.6413μg / mL and 2821.363μg / mL at 34.5 days (3.5 days).

Keywords


goose; immunoglobulin G; ELISA

Full Text:

PDF

References


Chen Xuelan, Xu Yang, Xiong Yonghua.Study on the immune response of ochratoxin A antigen in Chinese white rabbits and

Roman hens [J] .Hecifi c Research, 2003,1.

Jensenius J C, Andersen I, Hau J, et al. Eggs: convenience pack-aged antibodies. Mehtods for purifi cation of yolk IgG [J].

J-munol methods, 1981, 46 (1): 63-68

ROSE, M.EORORLANS, E. Immunocortintheegg, embryoandyoungchick. [J] Developmental and Comparative Immunology,

, 5 (1): 15-20

Sun Jianhong, Liu Baoquan. Development of chicken immunoglobulin detection technology [J]. Heilongjiang Animal

Husbandry 2000 (10): 26-27

Lundqvist ML, Middleton DL, Hazard S, et al. The ImmunoglobulinHeavy Chain Locus of the Duck: 'GENOMIC

ORGANIZATION ANDEXPRESSION OF D, J, AND CREGION GENES' [J]. J Biol, Chem., 2001, 276 50): 46729-46736

Liao Ming, Qiu Heying. Basic knowledge of poultry immunology (J). Chinese Journal of Veterinary Medicine, 1996,

Li Ding, Zhu Kuanyou, Bao Wenqi, et al. Anti-chicken Newcastle disease virus high egg yolk IgG development and application

[J] .Zhengzhou animal husbandry, 1990,10 (1): 1 ~ 3

Yang Yurong, Zheng Shimin. Isolation, extraction and identifi cation of yolk immunoglobulin [J]. FOOD SCIENCE u0026

TECHNOLOGY, 2003, (5): 99 ~ 101

Svendsen L, Crowley A, Ostergaard L H, et a1. Development and comparison of purifi cation strategies for chicken antibodies

from egg yolk [J]. Lab Anim Sci. 1995, 45 (1): 89 ~ 93

Xun Xixing. Veterinary Immunology [M]. Beijing: China Agricultural Publishing House, 1997,50

He Zhaoyang, Hu Guixue, Wang Chunfeng. Animal immunology experiment technology [M]. Jilin Science and Technology

Press, 2002

Polson A, Coetzer T, Kruger J, et al. Improvements in the isolation IgY from the yolks of eggs Jaid immunized hens [J]

.immunol invest, 1985, 14: 323 ~ 327

GU You-fang, LIU Yan-hui, CHEN Hui-liang et al. Advances in extraction and extraction of immunoglobulin and its

application [J]. Journal of Animal Husbandry and Veterinary Medicine, 2004,12: 12 ~ 14

Higgins D A, Cromie R L.Purijication of duck immunoglobulins: an evaluation of protein A and protein G affinity

chromatography [J]. Trans Immuno Immunopath, 1995, 44: 169-180

Ken T, Hirom I. Specifi c chicken egg antibody and method for its production. European Patent Aplication, EP 050329l A1

Polson A, von Wechmar MB, Fazakerley G.Antibody to proteins from yolk of immunized hens [J] .Immunological

communications, 1980, 9 (5): 495-514


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.