Anti-tumor Effect of Shikonin on S180 tumor - Bearing Mice

  • Guodong Cheng
Keywords: Shikonin, apoptosis, Caspase-3, 9


Objective: To investigate the changes of cell cycle and the content of P53 protein in S180 cells by using the specific fluorescent probe to identify the biological target to be measured according to the change of the fluorescence signal. The results showed that Shikonin had good anti-tumor effect. Methods: Cell cycle changes and P53 protein content in S180 cells were detected by flow cytometry. Results: With the increase of Shikonin dose, S180 cells G1 and S phase content gradually increased, it can be inferred Shikonin action in cells, and the cells can be blocked in G1 and S phase. With the increase of the dose of Shikonin, the expression of P53 protein increased. This suggests that Shikonin may up-regulate P53 activity and induce apoptosis. Conclusion: From the experimental results, we can see that Shikonin has a good antitumor effect in vivo.


Dong Zhiyong. New century goal of tumor prevention and treatment [J]. Chinese Journal of Cancer, 2002, 24: 311-312.

Martin, Zhou Jianfeng. Current status and comment on the development of clinical research of tumor gene [J]. Chinese Journal

of Cancer, 2003, 25: 1-2.

Yang J, Liu XS, Bhalla K, et al. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked [J],

Sicence, 1997, 275 (5303): 1129-1132.

Henartner MO. Death cycle and Swiss army knives [J]. Nature, 1998, 391 (6666): 441-442.

Raff M. Cell suicide for beginners [J]. Nature 1998, 396 (6707): 119-122.

Singh F, Gao D, Lebwohl M G, et al. Shikonin modulates Cell proliferation by inhibiting epidermal growth factor receptor

signaling in human epidermoid carcinoma cells [J]. Cancer lettr, 2003, 200 (2): 115-121.

Wu Zhen. Study on mechanism of apoptosis induced by shikonin in tumor cells [D]. Shenyang Pharmaceutical University,

Lu Dongdong. Topoisomerase and tumor research [J]. Journal of Qilu Journal of Cancer, 1997,4 (4): 302-303.

Xu Zhongli, Zhou Muying. Treatment of advanced cancer treatment concept and treatment of recent (continued) [J]. Journal of

Practical Medicine, 2002, 18 (6): 672-674.

Yu Jinming, Li Baosheng. Clinical application status and problems of intensive radiation therapy [J]. Chinese Journal of

Cancer, 2005, 27 (3). 188-190

Zhang Yijie, Tang Yan, Hu Xiangui, et al. Effect of oral chemical therapy on radical resection of pancreatic head cancer [J].

Chinese Journal of General Surgery, 2002, 17 (6): 352-353

Cao Shilong, Xu Liangzhong. Oncology new theory and new technology [M]. Shanghai: Science and Technology Education

Press, 1997, 267-286.

Sankawa U, Otsuka H, Kataoka Y, et al. Antitumor activity of sbikonin ,, alkannin and their derivatives [J]. Chem Pharm Bull,

; 29 (1): 116-222.

Wanderson. Clinical Oncology [M]. Beijing: Science Press, 2002, 10: 2-3.

Zhen Yongsu editor, anti-tumor drug research and development [M], Beijing, Chemical Industry Press, 2004: 26.

Louhuanjin. Body fluid cytology smear technique [J]. Zhejiang Cancer, 2000,6 (2): 86.

Liu Xiaowu. Production of standardized blood smear recommendations and research [J]. Chinese Journal of Hemorheology,

,17 (1): 130.

Qu Hongda. Effects of Buyang Huanwu Decoction Serum on P53 and P21 Expression in Rat Cortical Neurons after Hypoxia in

Vitro [J]. Chinese Journal of Integrated Traditional and Western Medicine, 2004, 24 (2): 133.

Original Research Article